Quick answer
The Serum Estrogen (Estradiol) Test costs ₹800 at Live Line Health Care. Measures estradiol, the main estrogen in women of reproductive age, to look at ovarian function, menopause, fertility treatment and some hormone problems in men. No fasting is needed. It uses a blood sample and the report is usually ready within 24 hours.
Also known as: Estradiol, Oestradiol, E2, Serum Estradiol, Serum Estrogen, Estrogen Test, Oestrogen Test, Day 3 E2, Serum Esrtogen
What is the Estradiol (E2) Test?
Estradiol, often written E2, is the strongest and most important of the estrogens in women between puberty and menopause. It is made mainly by the growing follicles in the ovaries, so its level climbs through the first half of the cycle, peaks just before ovulation, dips, then rises again in the second half. Estradiol shapes periods, the uterine lining, bone strength, skin and mood. Men make it too, in smaller amounts, mostly by converting testosterone.
Because estradiol changes so much from day to day, the result is only meaningful when the cycle day is known. A doctor usually orders it on day 2 to 5 together with FSH and LH to look at ovarian function, to explain missed periods or suspected early menopause, or to read an FSH result correctly. In fertility treatment it is checked repeatedly to follow how the ovaries respond to stimulation injections. In men, it can help explain breast enlargement or low libido.
What it measures: The level of estradiol (17-beta estradiol) in serum, reported in pg/mL. Some reports use pmol/L, where the number is about 3.67 times larger.
Parameters included (1)
- Estradiol (E2)
Why is this test done?
- Assessing ovarian function together with FSH and LH on day 2 to 5 of the cycle
- Investigating absent or irregular periods and suspected early menopause
- Monitoring the ovarian response during IVF or ovulation induction
- Checking estrogen levels on hormone replacement therapy, when advised
- Looking into early or delayed puberty in girls
- Evaluating breast enlargement (gynaecomastia) or low libido in men
When should you get tested?
- Periods that have stopped or become very irregular before the age of 45
- Hot flushes, night sweats, vaginal dryness or sleep problems
- Difficulty conceiving, or you are starting fertility treatment
- Low body weight, very intense exercise or an eating disorder with missed periods
- Breast swelling or tenderness in a man
How to prepare for the Estradiol (E2) Test
- 1No fasting is needed.
- 2Give the sample on the cycle day your doctor asks for, usually day 2 to 5, counting the first day of bleeding as day 1.
- 3During fertility treatment, follow your clinic’s timing exactly, as the result guides the next injection.
- 4Tell the staff about birth control pills, hormone therapy, fertility injections and biotin supplements, which can all change the result.
Estradiol (E2) Test normal range
| Parameter | Typical reference range |
|---|---|
| Women, early follicular (day 2 to 5) | About 20–80 pg/mL |
| Women, pre-ovulation peak | About 100–500 pg/mL |
| Women, luteal phase | About 40–250 pg/mL |
| Women, after menopause (not on HRT) | Usually below about 30 pg/mL |
| Adult men | About 10–40 pg/mL |
Estradiol ranges vary widely between analysers, and very low values (after menopause, in men and children) are measured less precisely by routine methods. Ranges are typical adult values and vary between labs, age and sex. Always use the range printed on your report and discuss results with your doctor.
Understanding your results
- A low estradiol together with a high FSH suggests the ovaries are not responding, as at menopause or with premature ovarian insufficiency if you are under 40.
- A low estradiol with low or normal FSH and LH points to reduced signalling from the brain, which can follow low body weight, intense training, stress or high prolactin.
- A high day 2 to 5 estradiol (often above about 80 pg/mL) can hide a raised FSH, so a normal FSH on the same day may look better than ovarian reserve really is. Fertility doctors read the two together.
- During IVF, estradiol rises with the number of growing follicles. Very high values can raise the risk of ovarian hyperstimulation, which your clinic watches for.
Health packages that include this test
The Estradiol (E2) Test is included in these full body checkups:
Estradiol (E2) Test – FAQs
What is the price of the Estradiol (E2) Test?
The Serum Estrogen (Estradiol) Test costs ₹800 at Live Line Health Care, at all 45 centres and for home sample collection.
Are estrogen and estradiol tests the same?
Mostly, yes. Estrogen is a family of hormones, and estradiol is the main one in women of reproductive age. When a doctor asks for a serum estrogen test, the lab usually measures estradiol. Other estrogens, such as estrone after menopause and estriol in pregnancy, need separate tests.
Which day of the cycle is best for an estradiol test?
Day 2 to 5 is most common, when estradiol is at its baseline and can be read with FSH and LH. Your doctor may choose another day, for example mid-cycle to look for a pre-ovulation rise, or a specific day during fertility treatment. Follow the timing on your prescription.
Why is estradiol checked so often during IVF?
Estradiol is produced by the follicles growing in response to injections, so its rise shows how the ovaries are responding. Together with scans, it helps the clinic adjust doses, time the trigger injection and lower the risk of overstimulation. That is why samples are timed precisely.
What does low estradiol mean after menopause?
After menopause, the ovaries make very little estradiol, so low values are expected and are not a problem in themselves. Symptoms such as hot flushes, vaginal dryness or bone loss are discussed with your doctor, who weighs treatment options. The test is not needed to diagnose menopause in most women.
Why would a man get an estradiol test?
Men convert some testosterone into estradiol. A doctor may check it if a man has breast enlargement, low libido, or is on testosterone treatment, where estradiol can rise. Liver disease, obesity and some medicines can also change it. The male range is much lower than the female range.
Where can I get the Estradiol (E2) Test done?
At any of our 45 Live Line Health Care centres in 40 cities across Andhra Pradesh and Telangana, including Hyderabad, Visakhapatnam, Warangal, Kakinada and Nellore, or book home sample collection.
Medical disclaimer: This information is for general awareness only and is not a substitute for professional medical advice. Please consult a qualified doctor to interpret your results and before starting or changing any treatment.